Journal: Stem Cell Research & Therapy

Article Title: Pyrroloquinoline quinone promotes human mesenchymal stem cell-derived mitochondria to improve premature ovarian insufficiency in mice through the SIRT1/ATM/p53 pathway

doi: 10.1186/s13287-024-03705-4

Figure Lengend Snippet: MSC-Mito and PQQ synergistically promote mitochondrial biogenesis while inhibiting DNA damage and cellular apoptosis. ( A ) Western blot analysis of SIRT1 and PGC-1α protein expression in mouse ovarian tissues of various groups. ( B ) Western blot analysis of pATM and TERF2 protein expression in mouse ovarian tissues of various groups. ( C ) Western blot analysis of the apoptosis-related proteins p53, Bax, and Bcl-2 expression in mouse ovarian tissues of various groups. ( D ) Western blot analysis of SIRT1 and PGC-1α protein expression in KGN cells. ( E-F ) qPCR analysis of SIRT1 and PGC-1α gene expression. ( G ) qPCR analysis of mtDNA copy number. ( H-I ) Comet assay to assess DNA damage. ( J-K ) Flow cytometry analysis to detect cellular apoptosis. ( L ) Western blot analysis of the impact of PQQ and MSC-Mito on the ATM/p53 signalling pathway in vitro. Scale bars: 10 μm. Values are the means ± SEMs. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Subsequently, the membrane was blocked using 5% skim milk for 1 h. Then, the sections were incubated overnight at 4 °C with the corresponding primary antibodies, including pATM, PGC-1α, TERF2 (ABclonal, China), SIRT1, p53 (Proteintech Group, China), Bax, Bcl-2, and β-actin (HuaBio, China), and then probed with secondary antibodies.

Techniques: Western Blot, Expressing, Single Cell Gel Electrophoresis, Flow Cytometry, In Vitro

Journal: Stem Cell Research & Therapy

Article Title: Pyrroloquinoline quinone promotes human mesenchymal stem cell-derived mitochondria to improve premature ovarian insufficiency in mice through the SIRT1/ATM/p53 pathway

doi: 10.1186/s13287-024-03705-4

Figure Lengend Snippet: The mechanism of action for MSC-Mito therapy in conjunction with PQQ in chemotherapy-induced POI is as follows: PQQ facilitates MSC-Mito proliferation in cells and, in synergy with MSC-Mito, mitigates the elevated ROS levels induced by chemotherapy, thereby reducing mitochondrial damage. Simultaneously, this enhances the expression of SIRT1, upregulates TERF2 to promote DNA damage repair, suppresses ATM/p53 pathway activation, and reduces cellular apoptosis. Furthermore, SIRT1 upregulates PGC-1α, promoting mitochondrial biogenesis and maintaining cellular homeostasis

Article Snippet: Subsequently, the membrane was blocked using 5% skim milk for 1 h. Then, the sections were incubated overnight at 4 °C with the corresponding primary antibodies, including pATM, PGC-1α, TERF2 (ABclonal, China), SIRT1, p53 (Proteintech Group, China), Bax, Bcl-2, and β-actin (HuaBio, China), and then probed with secondary antibodies.

Techniques: Expressing, Activation Assay